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The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR merchandise were being digested and cloned right into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A point mutation was launched by the quick modify internet site-directed